Localization of TMEM16B in mouse retina. A , Immunofluorescence microscopy on a cryosection of mouse retina labeled with TMEM16B-7H10. Differential interference contrast image on the left shows the different retinal layers. OS/IS, Outer and inner segments; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. B , Outer plexiform layer simultaneously stained with the TMEM16B-7H10 (red) and antibodies toward PSD95, VELI3, and synaptophysin. Yellow staining in the merged images (right) indicates colocalization. White arrows point to synaptic terminals with plasma membranes labeled by TMEM16B-7H10 (red) and synaptic vesicles stained by synaptophysin antibodies (green). Scale bars: 25 μm.