Interaction of TMEM16B with PSD95 and MPP4. A , Bacterially expressed GST (control) or GST fusion protein containing the C terminus of TMEM16B (GST+C-term) were incubated with lysates from 293-EBNA cells transfected with PSD95, VELI3, and MPP4. B , GST-pulldown assay performed with a TMEM16B deletion mutant lacking the C-terminal three amino acids (GST+C-termΔ3). C , GST (control) or GST+C-term fusion proteins were incubated with 293-EBNA cell lysates transfected with β-PSD95, MPP4 or cotransfected with MPP4 and α- or β-PSD95. A–C , Bound proteins were identified by immunoblotting with antibodies as indicated below the respective images. Inputs represent 2% of total cell lysates in all experiments.