Quantification of neurogenesis by the detection of BrdU/NeuN- and DCX-expressing cells. A, Immunodetection of BrdU in the dentate gyrus of wild-type mice (upper micrograph) and G-CSF^−/− mice (lower micrograph). G-CSF deficiency led to a significant reduction in the total amount of BrdU-positive cells (mean G-CSF^−/−, 1944 ± 253.31 SEM; mean wild type, 6873 ± 433.09; p < 0.001, Student's t test) and BrdU/NeuN-positive cells (mean G-CSF^−/−, 1634.11 ± 189.56 SEM; mean wild type, 5690.69 ± 319.77; p < 0.001, Student's t test). Scale bar, 80 μm. B, Immunodetection of DCX in the dentate gyrus of wild-type mice (upper micrograph) and G-CSF^−/− mice (lower micrograph). G-CSF deficiency led to a significant reduction in the amount of DCX-expressing cells compared with wild-type mice (mean G-CSF^−/−, 8004 ± 3011.07 SEM; mean wild type, 34,752 ± 11,317.28; p < 0.05, Student's t test). Scale bar, 80 μm