NOS1AP and Scrib colocalize to synaptophysin-positive presynaptic specializations in axons. A, B, Hippocampal neurons cultured for 16 DIV stained for Scrib (A) and the presynaptic marker VAMP (B). C, Merged image of A and B. Colocalization of endogenous Scrib with VAMP-positive puncta (arrows in A–C). Some Scrib-positive puncta are not VAMP positive (arrowheads in A–C). D, E, Images of primary hippocampal neurons following transfection with YFP-Scrib (D) and RFP-synaptophysin (E). F, Merged image of D and E. Scrib and synaptophysin colocalize in axons (arrows, D–F). G–J, NOS1AP (G) and Scrib (H) colocalize in synaptophysin (I)-positive puncta (arrows in G–J). Scale bars: A–F, 5 μm; G–J, 2.5 μm.