NOS1AP and Scrib colocalize in dendrites. A, Fluorescent image of a dissociated hippocampal neuron cultured for 16 DIV and stained with a Scrib antibody. B, C, Dissociated hippocampal neurons 16 DIV stained for Scrib (B) and phalloidin (C). D, Merged image of B and C. Note the colocalization of endogenous Scrib in dendritic spines (arrows in B–D). E, F, Dissociated hippocampal neurons 15 DIV stained for NOS1AP (E) and synaptophysin (F). G, Merged image of E and F. Note partial colocalization of endogenous NOS1AP and synaptophysin (arrows in E–G). H, I, Dissociated hippocampal neurons 13 DIV were transfected with myc-Scrib (H) and YFP-NOS1AP (I). J, Merged image of H and I. Scrib and NOS1AP colocalize in dendritic shafts (arrowheads in H–J) and in dendritic spines (H–J, arrows). K, L, NOS1AP (K) is seen at the tips of dendritic spines with actin (L) (arrows in K, L). M, Merged image of K and L. Scale bars: A, 15 μm; B–G, K–M, 2.5 μm; H–J, 10 μm.