The bursting properties of the e-pF neurons are altered at E14.5 in the Tshz3 mutant. A , Membrane trajectory (top traces) of an e-pF neuron recorded at two different resting potentials (left and right traces) recorded simultaneously with the e-pF population integrated activity (bottom traces). Seven superimposed bursts and the corresponding average trace (red) are illustrated at an extended time scale in the black box. The three bottom right panels represent the immunolabeling for PHOX2b (blue) for a biocytin-filled (green) e-pF neuron previously electrophysiologically identified with a patch-clamp recording. B , Same legend as in A for an e-pF neuron recorded from a Tshz3 mutant preparation. The neuron generates abnormal bursts, and the duration and the shape of the bursts are variable from one event to the other (see the box). Immunolabeling for PHOX2b (blue) and β-gal (red) ascertained the identity of the recorded neuron filled with biocytin (green).