mtHsp75 overexpression attenuates the loss of Dcx⁺ cells induced by CM exposure. A, Examples of Dcx staining in control plasmid- and mtHsp75-transfected cultures. Transfected cells were identified by DsRed2 coexpression (see Materials and Methods) and are indicated by arrows. Scale bar, 50 μm. B, Changes in the number of transfected Dcx⁺ cells in control (LXSN empty plasmid) and mtHsp75-overexpressing cultures induced by 24 h CM exposure (black columns). The data are representative of three independent experiments with a total of ∼150 Dcx⁺ cells per vector control condition. Shown are mean ± SEM. ^#p < 0.001 compared with respective plasmid- or mtHsp75-transfected cultures without CM treatment; **p < 0.01 compared with plasmid without CM treatment; ***p < 0.001 compared with plasmid plus CM.