MCHr1 antagonist reduced caloric intake in calorically restricted mice after high-fat diet withdrawal. A, C, E, During 3 d limited access to a high-fat pellet, mice previously calorically restricted (Rstr) show increased binge-like consumption of high-fat diet compared with controls (ad libitum) (^#p < 0.001). (A) but not differences in 24 h chow (C) or total calories (E). B, On the fourth day of testing, increased caloric intake of the high-fat pellet continued in the restricted mice (^##p < 0.01). However, there was no effect of drug on high-fat caloric intake. D, The MCHr1 antagonist, GSK-856464, reduced chow caloric intake only in previously CR mice (*p < 0.05). F, The MCHr1 antagonist, GSK-856464, significantly reduced overall caloric intake only in previously CR mice (*p < 0.05) (ad libitum-VEH, n = 7; ad libitum-GSK, n = 8; ad libitum-CIT, n = 8; Rstr-VEH, n = 8; Rstr-GSK, n = 8; Rstr-CIT, n = 8). Data are mean ± SEM.