CP-AMPARs are eliminated by NREM sleep discharge patterns. A, EPSP amplitude time course (mean ± SEM) in control and after EPSP burst pairings (■). The CP-AMPAR-selective inhibitor PhTx (5 μm) was bath applied toward the end of the experiment as indicated. Sample EPSPs above are in control (1), after conditioning (2), and in PhTx (3). A sample EPSP burst is illustrated (■). Bottom, V_m and R_i of a representative cell remained stable throughout the experiment. B, Representative EPSC current–voltage relationship and corresponding EPSCs obtained by repatching the neuron with a Cs⁺-based solution that contained 0.3 mm spermine. A straight line was fitted by linear regression. C, Summary histogram of RI (mean ± SEM) in control (black bar) and after conditioning (gray bar). **p < 0.0005. D, E, EPSP amplitudes (mean ± SEM) in control and after burst pairings (■). PhTx was bath applied toward the end as indicated. Sample traces are shown for control (1), after conditioning (2), and in PhTx (3). D, The tyrosine phosphatase inhibitor orthovanadate (1 mm) was bath applied throughout the experiment. LTD was still significant (p < 0.05), but PhTx was without effect (p > 0.5). E, The PP1/2A inhibitor okadaic acid (0.1 μm) was added to the pipette solution. Both burst pairings and PhTx led to a significant EPSP reduction (p < 0.005 and p < 0.05, respectively).