Exogenous BDNF decreases EPSCs in the nTS slice preparation. A, Representative current tracing from an individual nTS cell illustrating sEPSCs under control conditions (upper trace) and in the presence of BDNF (lower trace). Note the decrease in sEPSC frequency and amplitude with BDNF. B, Cumulative probability plots of sEPSC interevent intervals (n = 6, 10 ms bin) under control conditions and in the presence of exogenous BDNF, showing a significant rightward shift (decreased sEPSC frequency) induced by BDNF (p < 0.05, Kolmogorov–Smirnov test). C, Cumulative probability plots of sEPSC amplitude (n = 6, 2 pA bin) under control conditions and in the presence of BDNF, showing a significant leftward shift (reduced sEPSC amplitude) induced by BDNF (p < 0.05, Kolmogorov–Smirnov test). D, Representative TS-EPSCs (average of 20 traces) under control conditions (aCSF, left trace) and in the presence of BDNF (right trace). Bath application of BDNF reduced the amplitude of TS-evoked EPSCs at 0.5 Hz. Cells were voltage-clamped at −60 mV. Arrows indicate stimulus artifact. E, Comparison of TS-EPSC amplitude (n = 6) in controls (open bar) and during BDNF (black bar) application. Small, connected symbols indicate data from individual neurons. Larger symbols indicate mean data. Note the reduction in amplitude in the presence of BDNF (*p < 0.05, paired t test).