BDNF decreases nTS action potential discharge. A, Representative traces of neuronal discharge under control conditions (left) and in the presence of BDNF (right) in response to 30, 40, and 50 pA (500 ms) current injection steps (n = 6) in an individual nTS cell. Note that increasing depolarizing current injection increased action potential discharge. However, APD was less following application of BDNF. B, Group comparison of APD of cells at resting membrane potential in response to current injection steps ranging from 10 −100 pA. Note the decreased APD in the presence of BDNF at currents ≥ 40 pA (*p < 0.05 vs control, two-way repeated-measures ANOVA).