Dock7 knockdown in mice changes Rho GTPase signaling in sciatic nerves. A, B, D, Seven-day-old sciatic nerve tissues were lysed, affinity precipitated, and immunoblotted with an antibody against each small GTPase. Total small GTPase is also shown. C, Sciatic nerve tissue extracts were immunoprecipitated with an anti-JNK1 antibody and immunoblotted with an antibody against (pThr183/pTyr185)JNK. Total JNK1 is also shown. E, Sciatic nerve tissue extracts were immunoblotted with an antibody against (pT853)MBS or MBS. TG, Transgenic mice; NTG, nontransgenic mice.