N-cadherin knock-out mice display a slight delay in myelination without impacting SC numbers, ensheathment, myelin structure, and compaction. A, Western blot analysis was performed using mouse sciatic nerves from SC-specific knock-out mice (Dhh-Cre^+/−, Flox-N-cad/Flox-N-cad) and littermate controls (Dhh-Cre^−/−, Flox-N-cad^+/−). Westerns were probed for N-cadherin, Oct-6, MBP, P0, and β-actin (loading control). B, Myelination in knock-out and control mice was quantified as the number of myelinated axons per square millimeter. The black columns represent the knock-out nerves, and the gray columns are the littermate controls. Nerves from three separate mice were analyzed, and the values are displayed as the mean number of myelinated axons ± SD. Significance was evaluated using Student's t test (*p < 0.05). C, The number of Schwann cells, axonal sorting, compaction, and extent of myelination were examined by electron microscopy of knock-out and control mouse sciatic nerves at 0, 5, 15 d postnatal and adult nerves.