A, The corpus callosum analysis in Slc12a6 KO lines. Both Slc12a6 conditional KO lines were analyzed using gold chloride staining of mid-sagittal cut of the brains and compared with their respective wild-type littermates (only one shown) at 8 weeks of age. B, A representative WT brain that was stained by gold chloride is shown indicating the parameters to measure the CC and AC at midline sagittal plane. C, Cortical neurons were cultured from Slc12a6^Δ18/Δ18 and WT embryos at E15.5, and the lengths of axons were compared.