A, AngII infusion increased ET1 in pial cerebrovascular preparations at 14 d. SR49059 (in drinking water, days 7–14) prevents the elevation in ET1 (*p < 0.05 form sham; n = 5–6 per group). B, AngII infusion increases ECE-1 mRNA in pial microvascular preparations at 14 d (*p < 0.05 from Saline and 7 d; n = 5 per group). C, ET1 immunoreactivity is increased by AngII infusion in pial arterioles of the somatosensory cortex at 14 d. The endothelial marker CD31 was used to identify the vessels. D, Immunogold electron microscopy demonstrates ET1 in endothelial cells (arrows). AngII infusion (14 d) increases the immunogold particles (D).