LM11A-31 increased the number of myelinated axons by twofold at 100 mg/kg. A–D, Representative images of the myelin profile in the dorsal column after toluidine blue staining. The boxed areas from the most dorsal regions in the midline in A–D are also shown at the center (white-lined box). The black-lined boxes in A indicate the areas selected for quantification of myelinated axons. E, The average number of myelinated axons in the box increased by twofold with 100 mg/kg LM11A-31 compared with that with 0 mg/kg samples; p values were obtained by Student's t tests.