Inhibitory effect of the CBS inhibitor AOAA on gastric sensitivity. A, Representative EMG recordings from CON and diabetic rats responding to graded GD at 60, 80, 100, and 120 mmHg 4 weeks after vehicle (CON) or STZ injection. B, AUC of EMG recordings from the STZ group (n = 7) was significantly higher than CON (n = 7) at distention pressures of 60–120 mmHg (*p < 0.05, **p < 0.01, 2-sample t test and Mann–Whitney test after Friedman ANOVA). C, Representative EMG recordings from diabetic rats in response to graded GD 60 min after a single injection of AOAA at doses of 2.5, 5, and 10 mg/kg body weight. D, AOAA at doses of 5 mg/kg (n = 7) and 10 mg/kg (n = 5) dramatically suppressed EMG amplitude, whereas NS (n = 5) and AOAA at 2.5 mg/kg (n = 5) had no significant effect on EMG (*p < 0.05 compared with NS at each pressure, Tukey post hoc test after one-way ANOVA). E, Representative EMG recordings from diabetic rats in response to graded GD at different distention pressures between 60 and 120 mmHg after AOAA (5 mg/kg) treatment. F, Bar graph showing that administration of AOAA resulted in a significant reduction on EMG responses of diabetic rats 60 min at 80, 100, and 120 mmHg distention pressures and 30 min at distention pressure of 120 mmHg (n = 7, *p < 0.05 compared with before treatment, Dunn's post hoc test after Friedman ANOVA).