Increase in excitability by NaHS, a donor of H₂S, applied at 250 μm to gastric-specific DRG neurons from healthy female CON rats mimics the effect of CBS in producing H₂S. A, Application of NaHS significantly depolarized RPs (n = 10, **p < 0.01 compared with before treatment, one-way repeated-measures ANOVA). B, Application of NaHS did not markedly alter R_in (n = 10, p > 0.05 compared with before treatment, one-way repeated-measures ANOVA). C, NaHS markedly reduced rheobase of DiI-labeled DRG neurons (n = 10, **p < 0.01 compared with before treatment, one-way repeated-measures ANOVA). D, NaHS application decreased AP threshold of DiI-labeled DRG neurons (n = 10, *p < 0.05 compared with before treatment, one-way repeated-measures ANOVA). E, F, NaHS also greatly increased numbers of AP evoked by 2× and 3× rheobase current stimulation (n = 10, *p < 0.05 compared with before treatment, Friedman ANOVA followed by Dunn's post hoc test). Note that all of these effects are washouts.