TRPA1 promotes scratch-dependent and scratch-independent expressional changes in the skin. A, Venn diagram displaying relative numbers of scratch-dependent, TRPA1-dependent, and scratch- and TRPA1-dependent genes significantly modulated in WT AEW mouse cheek skin. B, Analysis of a subset of genes that were previously implicated in processes related to chronic itch to determine dependence on scratching and/or TRPA1 (Fig. 5A). Green indicates AEW-evoked increase in expression; magenta, AEW-evoked decrease in expression. C, Venn diagram displaying relative numbers of scratch-dependent, TRPA1-dependent, and scratch- and TRPA1-dependent itch-related genes significantly modulated in WT AEW mouse cheek skin.