Microarray analysis of Dgcr8^+/− mouse hippocampus identified candidate genes for 22q11.2 deletion-associated schizophrenia. Gene expression microarray analysis was performed on mRNA isolated from the hippocampus of 2-month-old male Dgcr8^+/− and Dgcr8^+/+ mice. A, Heat map representing hierarchical clustering of Dgcr8^+/− and Dgcr8^+/+ mice hippocampus cDNA microarray data. Red and green squares represent expression levels greater than or less than the mean, respectively. Black squares represent mean expression levels. B, Scatter plot depiction of candidate genes involved in 22q11.2 deletion-associated schizophrenia from the microarray comparison of the hippocampus of Dgcr8^+/− and Dgcr8^+/+ mice. C, Quantitative PCR analysis showing significant downregulation of Ace, Klotho, Ttr, Sulf1, Htr2c, Igf2, and Otx2 gene expression in the hippocampus of Dgcr8^+/− mice (***p < 0.001, Dgcr8^+/− vs Dgcr8^+/+, two-tailed t test, n = 4, mean ± SEM). D, Western blotting analysis of IGF2 expression in Dgcr8^+/+ and Dgcr8^+/− mice hippocampus. Top shows a reduced amount of IGF2 protein in the Dgcr8^+/− mouse brain, and bottom shows a similar amount of Actin protein.