cGMP signaling in ASI promotes quiescence and inhibits dauer formation. A, Expression of wild-type DAF-11 in ASI (daf-11 ASI) rescued the satiety quiescence defect of a daf-11 mutant. n = 10 for each group per experiment, repeated three times. **p < 0.01 by Student's t test. B, Addition of 8-Br-cGMP (1 mm, daf-11 + cGMP) only during refeeding period rescued the satiety quiescence defect of a daf-11 mutant. n = 10 for each group per experiment, repeated three times. **p < 0.01, ***p < 0.001, by ANOVA followed by Student's t test. C, Addition of 8-Br-cGMP (1 mm) enhances satiety quiescence under nonfasted conditions. Individual worms were placed singly on HB101-seeded plates for 18 h from L4, and then the quiescence durations were measured. n = 10 for each group, repeated three times. *p < 0.05, by Student's t test.