No PKA-dependent changes in inhibitory synaptic strength in the hippocampal CA1 region of WT and Syt12 KO mice. A, Measurements of inhibitory spontaneous mIPSCs reveal that there is no difference in the mIPSC frequency or amplitude between WT and Syt12 KO neurons obtained from littermate mice. Representative traces are depicted on top, and cumulative probability plots of the mIPSC frequency (left) and amplitude (right) at the bottom. B, The adenylate cyclase activator forskolin (20 μm) produces a similar enhancement of mIPSC frequency in WT and Syt12 KO hippocampus. Representative traces are shown on top, and a plot of the normalized mIPSC frequency as a function of time after forskolin application is shown on the bottom. C, iLTD is similarly induced in WT and Syt12 KO neurons. Representative traces are shown on top, and summary graphs of the normalized IPSC amplitude on the bottom. Numbers in the representative traces correspond to the indicated times in the summary. iLTD was triggered using TBS at time 0, as indicated by the arrow. D, Forskolin (20 μm) produces a similar acute enhancement of evoked IPSCs in WT and Syt12 KO hippocampus. Representative traces are shown on top, and summary graphs of the normalized IPSC amplitude as a function of time after forskolin addition is shown on the bottom. Data are plotted as means ±SEM. Number of slices/mice tested are indicated in parenthesis. No statistically significant differences between WT and Syt12 KO mice were detected in any of the parameters analyzed here.