p75NTR RNA oscillates in a circadian manner in the SCN and liver. Top, Schematic representation of the light schedule. Mice were entrained in a 12 h light/dark cycle and were killed at 4 h intervals (n = 4 per group). Bottom, At the indicated times, the SCN (left) and liver (right) were dissected; RNA was extracted; and the levels of p75NTR, Per1, and Per2, together with nonoscillating control genes such as Clock for the SCN and Rorα for the liver, were analyzed by real-time PCR. The results of at least three independent experiments with duplicate measurements are shown. Data from CT20 are double plotted. Error bars represent the SEM of the mean, where the maximum RNA amount was set to 100. CT, Circadian time.
