ChAT–ChR2–EYFP mice have deficits in attention. The 5-CSRT task was used to measure attention in the ChAT–ChR2–EYFP mice. A, Mean response accuracy during probe trial sessions. B, Rate of omissions. To assess response patterns, both premature (C) and perseverative (D) responses were monitored during probe trials. E, Mean response latency. F, Mean reward collection latency. *p < 0.05, ***p < 0.001, n = 7 for both genotypes.