Imaging network responses to synthetic and natural sounds. a, In vivo two-photon micrograph of a single optical plane in A1 of a virgin mouse after bolus loading of Fluo-4 AM (green) and SR101 (red). Some of the cells are circled and numbered. This optical plane is 362 μm below the pia. Scale bar represents 20 μm. b, Relative changes in fluorescence (ΔF/F) of the seven neurons shown in a during presentation of a stimuli series (data not shown). Scale bars as in e. c, Single trial and mean responses of the seven neurons from a and b to eight different stimuli. Rows mark neurons 1–7 from top to bottom and columns mark different stimuli (annotated on the bottom). Each panel represents the responses to all trials of the specific stimulus (black) and its mean (red). *Panels that show a significant response. Scale bars as in f. d–f, Same as a–c, but from a lactating mother.