RFA and CFA as identified by OSM and ICMS. A, OSM and ICMS results in an animal. OSM was performed in the boxed region. The contour lines at 10% of the maximal amplitude of contralateral forelimb movement as determined by OSM are shown. The dots indicate the sites where ICMS induced contralateral forelimb movement (black), whisker movement (magenta), or no movement (cyan). The current amplitudes were <200 μA and >200 μA at the large and small dots, respectively. The green star indicates the bregma. B, RFA, CFA, SFA, and the imaged fields. Overlaid red and blue contours show the RFA and CFA, respectively, as revealed by OSM (n = 4 mice). Two maps acquired in the right hemisphere are mirror-reversed. Orange contours show SFA as determined by flavoprotein imaging (n = 3 mice). The contour lines at 50% of the maximal response to 1 s vibration of the contralateral forelimb are overlaid. Circles indicate the centers of the two-photon calcium imaged fields during the task. Three closed circles show the centers of the imaged fields that were confirmed to be the RFA or CFA by OSM after the imaging experiment. C, Effects of muscimol injection into the left hemisphere of the RFA (red), CFA (blue), or somatosensory barrel cortex (green) on lever-pull task performance. Muscimol was injected into each indicated area in three animals. The number of successful lever pulls in the 5 min after the injection was normalized to the number of successful pulls in the 5 min before the injection.