Characteristics of pull cells and post-pull cells. A, An example of a pull cell whose mean ΔF/F amplitude during the lever-pull period was proportional to the duration of the lever pull, Tpull. The Spearman's rank correlation coefficient was 0.52 (p < 10−9, Spearman's rank correlation coefficient test). B, Percentage of pull cells, post-pull cells, and non-task-related cells whose mean activities during the lever-pull periods were significantly correlated with the durations of the lever pull, Tpull (p < 0.05 by the Spearman's rank correlation test). Data were summarized from all 68 imaged fields (21 mice). C, Fluorescent traces of a post-pull cell that significantly responded to a 1 s vibration of the right forelimb were aligned with the start of successful lever pulls or the vibrations. Black lines and gray shading indicate mean ± SEM. The lever trajectories and sensory stimulation time are shown below. D, The percentage of cells that exhibited calcium transients that were significantly responsive to a 1 s vibration of the right forelimb. The mean transients 1 s before the stimulation and 1 s during the stimulation were compared using the Wilcoxon signed-rank test, p < 0.05. E, An example of a post-pull cell demonstrating calcium transients only in successful trials (and not in error trials). Black thick line indicates the mean trace. The lever trajectories in individual trials are overlaid in the bottom trace. F, An example of a post-pull cell demonstrating calcium transients in both successful and error trials. G, The percentages of lever return-related cells among the different cell types. H, The percentage of licking-related cells among the different cell types.
