
Spatial clusters of pull cells. A, tCCs relative to cellular distances for 11,536 pairs of pull cells (cyan circles) and 2151 pairs of post-pull cells (magenta triangles) across 48 fields that included ≥3 pull cells and ≥3 post-pull cells (21 mice). Regression lines are shown in the same colors. Cell pairs were grouped every 25 μm. ***p < 0.0001 and *p < 0.05 by the Wilcoxon rank sum test. For tCC values >175 μm, p > 0.05. B–D, Three examples of imaged fields. The number of primary-clustered cells and the diameter of the primary cluster are indicated at the top of each field. Black, green, blue, magenta, yellow, and gray circles indicate primary-clustered pull cells, other clustered pull cells, nonclustered pull cells, post-pull cells, other task-related cells, and non-task-related cells, respectively. Dotted circles surround the primary clusters. B is the same field as in Figure 4A. Pull cells in B were used for the analyses in Figures 7, B and C, and 9, A and B. E, Histogram of the diameters of the primary clusters (n = 36 fields that included the primary cluster from 18 mice). F, tCCs relative to cellular distances for 1075 pairs of primary-clustered cells (black squares) and 1680 pairs of nonclustered cells (blue circles) across 30 fields that included the primary cluster and ≥3 nonclustered cells (16 mice). Regression lines are shown in the same colors as the cluster types. **p < 0.001 and ***p < 0.0001 by the Wilcoxon rank sum test. tCC was not significantly different at distances of >75 μm (p > 0.05). Cell pairs were grouped every 25 μm.




