Inhibition of cannabinoid reuptake causes spillover 2-AG suppression of GABA release. A, Blocking endocannabinoid reuptake with the transporter blocker OMDM-2 (OMDM) caused a decrease in mIPSCs frequency, which was blocked by the CB1 receptor antagonist SR141716 (SR + OMDM) in SON neurons from normally hydrated rats. In SON neurons from dehydrated rats, blocking endocannabinoid reuptake (OMDM) had no effect on mIPSC frequency, and subsequent blockade of CB1 receptors caused an increase in mIPSC frequency (SR + OMDM). B, The decrease in mIPSC frequency caused by blocking endocannabinoid reuptake (OMDM) was inhibited by blocking 2-AG synthesis with THL (THL + OMDM); *p < 0.05.