The KCNQ2 allosteric activator ICA-27243 inhibits AP firing in Kcnq3 cKO but not Kcnq2 cKO mice. A, Voltage responses to various current injection steps (1 s) in pyramidal neurons from either control, Kcnq2 cKO, or Kcnq3 cKO mice. Membrane potential was kept at −60 mV by injecting a small DC current through the recording pipette. Representative traces showing the effects of deleting either Kcnq2 (red) or Kcnq3 (blue) on pyramidal neuron excitability and in the presence or absence of ICA-27243 (25 μm). B, Summary graphs showing the effect of ICA-27243 on pyramidal neuron AP number in control (n = 6), Kcnq2 CKO (n = 7), or Kcnq3 cKO (n = 6) mice. Data shown are means ± SEM. Statistical significance was determined by two-way repeated-measures ANOVA (*p < 0.05).