Phosphorylation of GluA1 at the Ser845 site is enhanced in the insular cortex after nerve ligation. A, Representative Western blots for phosphorylation of GluA1 at the Ser845 site in the PSD fraction on day 7 after nerve ligation. B, Representative Western blots for phosphorylation of GluA1 at the Ser831 site in the PSD fraction on day 7 after nerve ligation. C, In the PSD fraction, phosphorylation of GluA1 at the Ser845 site, but not at the Ser831 site, was significantly increased on day 7 after nerve ligation (n = 5 mice for each group). D, Representative Western blots for phosphorylation of GluA1 at the Ser845 site in the non-PSD fraction on day 7 after nerve ligation. E, Representative Western blots for phosphorylation of GluA1 at the Ser831 site in the PSD fraction on day 7 after nerve ligation. F, In the non-PSD fraction, phosphorylation of GluA1 at the Ser845 site was significantly decreased on day 7 after nerve ligation (n = 5 mice for each group). Phosphorylation of GluA1 at the Ser831 site showed no change (n = 5 mice for each group). *p < 0.05 compared with sham control. S, Sham; N, nerve ligation.