AC1 determines the upregulation of synaptic GluA1 and translocation of AKAP79/150 and PKA in the insular cortex. A, On day 7 after nerve ligation, the abundance of GluA1 and its phosphorylation at the Ser845 site in the PSD fraction from AC1^−/− mice with nerve ligation showed no change compared with sham control (n = 4 mice for each group). B, No upregulation of AKAP79/150, PKA RIIβ, or PKA Cα was observed in the PSD fraction of the insular cortex from AC1^−/− mice with nerve ligation compared with sham control (n = 5 mice for each group). C, In the non-PSD fraction of the insular cortex from AC1^−/− mice, the abundance of AKAP79/150 or phosphorylation of GluA1 at the Ser845 site showed no change on day 7 after nerve ligation compared with sham control (n = 5–6 mice for each group).