Axonal sprouting and mTOR dependency in young adult mice. A, Schematic representation of spinal cord section showing two areas (Mid and Z1) where vertical lines were drawn, and crossing axons were counted. B–H, Representative of C5 coronal sections, enlarged midline (B–H) or Z1 (B′–H′) regions from sham-operated Rosa-tdTomato (sham), pyramidotomized Rosa-tdTomato (Px only), pyramidotomized PTEN^f/fRosa-tdTomato with vehicle (Px+PTEN KO), or with rapamycin (Px+PTEN KO+Rap), and pyramidotomized Rosa-tdTomato-ChABC mice with vehicle (Px+ChABC) or rapamycin (Px+ChABC+Rapamycin); n = 8–13/group. I, Quantifications of crossing axons counted in different regions normalized against the numbers of labeled CST axons in sham-operated Rosa-tdTomato (sham), pyramidotomized Rosa-tdTomato (Px only), pyramidotomized PTEN^f/fRosa-tdTomato with vehicle (Px+PTEN KO) or with rapamycin (Px+PTEN KO+Rap), and pyramidotomized Rosa-tdTomato-ChABC mice with vehicle (Px+ChABC) or rapamycin (Px+ChAB + Rapamycin); *p < 0.001; #p < 0.05, two-way repeat measures ANOVA followed by Bonferroni post-test. Error bars indicate SEM; n = 8–13/group. Scale bar, 100 μm.