Postsynaptic GABA_A receptors on hypothalamic kisspeptin neurons are not saturated by the amount of GABA released during an mPSC. A, C, Representative mean mPSC amplitude (left) and normalized mean amplitude (right) under control conditions (black) and in the presence of diazepam (red) in arcuate nucleus (A) and AVPV (C) kisspeptin neurons. B, D, Mean ± SEM effects of diazepam reported as % change in arcuate nucleus (B) and AVPV (D) kisspeptin neurons. fwhm, Full-width at half-maximum; freq, frequency; int, interevent interval; amp, amplitude. N is reported as the number of recordings/number of animals: arcuate 6/6, AVPV 3/3.