IL-1R1^KO mice do not have increased myeloid cells in the blood and brain after RSD. WT and IL-1R1^KO C57BL/6 mice were subjected to RSD or left undisturbed as CON mice. Spleen, blood, and brain CD11b⁺ cells were collected 14 h after the final cycle of RSD. A, Average spleen weight. B, Average percentage of blood CD11b⁺ cells. C, Average percentage of blood monocytes (CD11b⁺/Ly6C^hi). D, Representative bivariate dot plots of CD11b/CD45 labeling in each experimental group. E, Average proportion of brain macrophages (CD11b⁺/CD45^hi). Bars represent average ± SEM. *p < 0.05, different from CON; ^#p < 0.10, different from CON.