Central NPS and NPSR1-A administration attenuate the anxiety phenotypes of HAB and LAB rodents. A–C, Intracerebroventricular NPS administration exerted an anxiolytic effect in rNABs in the LDB (A); in rHABs in a dose-dependent manner, with 1 nmol, but not 0.1 nmol, increasing the time an rHAB spent on the open arms of the EPM (B); and in mHABs compared with vehicle treatment (C). D, E, Contrastingly, intracerebroventricular administration of D-Cys(tBu)5-NPS (10 nmol), an NPSR1-A, increased the anxiety of rLABs (D) and mLABs (E) on the EPM. F–H, Intracerebroventricular NPS administration differentially affected locomotor activity with unchanged number of line crosses of rNABs in the LDB (F), increased closed arm entries of rHABs on the EPM (G), and unchanged distances that mHABs traveled on the EPM (H). I, J, D-Cys(tBu)5-NPS did not affect locomotor activity in either rLABs (I) or mLABs (J). Data represent the mean ± SEM. Numbers in parentheses indicate group size. *p < 0.05, **p < 0.01, ***p < 0.001, compared with vehicle group. Veh, Vehicle.
