Table 6.
List of primers used for sequencing of the mouse Nps gene with location and exons/promoter/downstream enhancing region
| Sequential order | Location | Orientation | Primer sequence (5′ → 3′) |
|---|---|---|---|
| −4 | Promoter | (+) | CCAGGCTTCCAGCTTGGCAC |
| −4 | Promoter | (−) | GCTGCTATTGCTGCTGTTTCTGAAG |
| −3 | Promoter | (+) | GGGTATCTTTGCCCTCCAAAAGGTG |
| −3 | Promoter | (−) | GGCAATCTGTTGTCACTGGTCCCTG |
| −2 | Promoter | (+) | TCCCTGCTCAACACCCCAAACC |
| −2 | Promoter | (−) | ACTGGTTGGCCTGGCTGTGG |
| −1 | Promoter | (+) | GAGGCTCCTGGCCACCCATG |
| −1 | Intron 2 | (−) | GGGCCCTCCACCATCCTGATCA |
| 1 | Promoter | (+) | TGGCAAGCTCTGAGTGAAGTCAACC |
| 1 | Intron 2 | (−) | TTTGGGCCCTCCACCATCCTGA |
| 2 | Promoter, Exon 1 | (+) | CCCATCTGCGCAGGTCTCGG |
| 2 | Intron 2 | (−) | TCCACTGTGCGGGTTTTTGGT |
| 3 | Promoter, Exon 1 | (+) | CATCTGCGCAGGTCTCGG |
| 3 | Intron 2 | (−) | CCAGAGTTACCTACTGTCACATAC |
| 4 | Intron 2 | (+) | AGCCGGTGGTAGCCCTACACT |
| 4 | Exon 3 | (−) | ACTCTGAGCCCGTTAGGAGAAGGG |
| 5 | Exon 3 | (+) | CCTTTCGCAACGGAGTCGGCT |
| 5 | Exon 3 | (−) | CGAGCCCTTGCTGCAGGTACC |
| 6 | Exon 3 | (+) | GTGCCACCAAGTGCAGTGGC |
| 6 | DER | (−) | GCTGGTGACCAAGGACAGGGT |
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DER, Downstream enhancing region.
