Selective Breeding for High Anxiety Introduces a Synonymous SNP That Increases Neuropeptide S Receptor Activity

Table 8.

List of primers used for allele specific quantification of HAB and LAB alleles in F1 offspring of rats and mice

Species, gene, specificity Orientation Primer sequence (5′ → 3′)
Rat, Npsr1, common (+) ACTGTGGCCAGACGACTCCT
Rat, Npsr1, rHAB-specific (−) CCTCGGTTGTAGCTGCAGCATATC
Rat, Npsr1, rLAB-specific (−) CCTCGGTTGTAGCTGCAGCATACT
Mouse, Npsr1, common (+) GTGCTGTTCTCCACGTGCAG
Mouse, Npsr1, mLAB-specific (−) TCAGGGGCCATGAAGTCTCGT
Mouse, Npsr1, mHAB-specific (−) TCAGGGGCCATGAAGTCTCGC
  1. The Journal of Neuroscience vol. 35 no. 11 4599-4613
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