Maternal n-3 PUFA condition does not influence retinal axonal projections to the brain. Representative coronal sections of Stage 45 tadpoles from frogs fed the (A–D) n-3 PUFA-adequate (Ade) or (A′–D′) n-3 PUFA-deficient (Def) diet for 60 weeks and immunostained with a neurofilament-associated protein antibody (green) illustrate the normal projection patterns of retinal axons (A, A′) out of the eye and along the optic nerve (on), and (B, B′) at the level of the optic chiasm (oc). Projection of axonal fibers along (C, C′) the optic tract (ot) and (D, D′) the rostral tectum (tect) were also similar in Stage 45 tadpoles from both groups. Counterstaining with DAPI (blue) reveals normal size and cellular organization in the (A, A′) retina, and (B–D, B′–D′) brains of tadpoles from frogs fed the adequate (A–D) and deficient (A′–D′) diets. rgc, Retinal ganglion cell layer; v, ventricle. Dorsal is up, ventral is down. Scale bar, 50 μm.