VU0463271 caused unremitting recurrent discharges in the low-Mg²⁺ model of epileptiform activity. A, Representative traces of 0 Mg²⁺-induced SLEs recorded from layer III of the medial entorhinal cortex in acutely prepared slices in the absence (top trace) and presence (10 μm) of VU0463271. The arrow in the bottom trace indicates the onset of VU0463271 perfusion. B, Higher-magnification traces of the first and last SLEs from the bottom trace in A (black bars). Note that the last SLE did not terminate. C, Representative traces demonstrating the effect of VU0463271 (100 nm, preincubated for 15 min) on 0 Mg²⁺-induced epileptiform activity.