Intrahippocampal administration of VU0463271 caused epileptiform activity in vivo. A, Representative recording of EEG activity before and after microinfusion of 100 μm VU0463271 (asterisk) into the hippocampus. B, An expanded trace of activity before and after microinfusion of VU0463271. C, Left, A representative spectrogram of the power of the EEG activity before and after VU0463271 microinfusion (asterisk). Right, The average power of the EEG activity demonstrates an increase in the low-frequency activity after VU0463271 microinfusion (red) compared with baseline (black).