Expression of A53T via lentiviral vector. A, Structure of lentiviral vector expressing A53T under the control of the human ubiquitin promoter. B, Cultured mouse brain cortical and striatal neurons transduced by lentiviral A53T showing positive staining by anti-α-syn (red). The nuclei of cells were stained by Hoechst dye (blue). Scale bar, 20 μm.