Retrograde transport of α9-GFP in axons is mediated by dyneins. A, Neurons were cotransfected with α9-GFP and p50-HA. Scale bar, 30 μm. B, Axons were identified with Neurofascin live staining. C, D, Inhibiting dynein function resulted in an abolishment of retrograde transport but increased immobile vesicles ***p < 0.001; **p < 0.01.