Direction of axonal integrin transport in mature CNS axons is controlled by ARF6. A, ARF6 was present in axons and dendrites of neurons at both 2 and 14 DIV. B, ARNO is increased in axons and dendrites of 14DIV neurons compared with 2 DIV and is expressed throughout the axon (MAP2 negative). C, Rab11 is present in dendrites but not in axons of 14 DIV neurons. D, For live imaging, neurons were cotransfected with α9-GFP and either ACAP1-myc or ARNO-E156K-myc and axons were identified with neurofascin. E, F, G, Kymographs and analysis of α9-GFP vesicle traffic in control axons or after transfection with ACAP1, ARNO-E156K, or ARNO-E156K combined with taxol treatment. ***p < 0.001; **p < 0.01; *p < 0.05.