Optogenetic stimulation of either global AcbSh neurons or selective AcbSh-LH terminals facilitates depressive-like behavioral despair. A, Optogenetic stimulation reduced the latency to first immobility and (B) increased the total time spent immobile during forced swim testing (C). All animals showed a negative correlation between latency and total immobility measures. D, There was no effect of global AcbSh neuron stimulation on general locomotor activity; n = 4–9/group.