Depletion of ER Ca²⁺ stores triggers SOCE in retinal Müller glia. A, Bright-field image of representative, acutely dissociated mouse Müller glial cells. Subregions are indicated with capital letters: D, distal stalk; S, soma; P, proximal stalk; Ef, end-foot. Scale bar, 10 μm. B, Ca²⁺ imaging. Representative calibrated [Ca²⁺]_i trace evoked during store depletion in Ca²⁺-free saline supplemented with CPA. SERCA inhibition elicited a small transient increase in cytosolic Ca²⁺ (white arrow) followed by store-dependent elevation in [Ca²⁺]_i during the re-exposure to 2 mm Ca²⁺, consisting of a peak response (black arrow) followed by a sustained plateau. C, Comparison of depletion-evoked [Ca²⁺]_i elevations in control conditions (black trace; n = 28, N = 4) and the presence of an inhibitory cocktail containing 2-APB (100 μm), SKF-96365 (10 μm), and Gd³⁺ (10 μm; red trace; n = 28, N = 4). D, Summary graph; average [Ca²⁺]_i ± SEM. N.S., Nonsignificant. **p < 0.01.