Ablation of TRPC1/3 channels enhances gliosis in an acute mouse glaucoma model. A, Injection of polystyrene microbeads (10 μm) elevates IOP in WT and Trpc1/3^−/− eyes. Averaged time course of intraocular pressure in wild-type and TRPC1/3^−/− mice. Time point of microbead injection is referred to as a day 0. B, Representative retinal sections prepared from wild-type or TRPC1/3^−/− mice and labeled for GFAP 8 weeks after the injection of microbeads. C, Averaged time course of the intensity of GFAP immunosignals in retinal sections from wild-type and TRPC1/3^−/− mice.