Normal ethanol-induced sedation requires DopEcR expression in the adult. A, B, Ethanol-induced sedation in the presence and absence of a ubiquitously expressed DopEcR-RNAi (A) or DopEcR cDNA (B). Left, Percentage of flies exhibiting sedation. Right, ST50. da>RNAi (UAS-DopEcR-RNAi/+;da-GAL4/+, n = 13) significantly increased resistance to ethanol sedation compared with RNAi (UAS-DopEcR-RNAi/+, n = 14) and da-GAL4 (da-GAL4/+, n = 12) controls (A). da>cDNA (da-GAL4/UAS-DopEcR-cDNA, n = 9) showed a trend to increase sensitivity to become sedated compared with cDNA (UAS-DopEcR-cDNA/+, n = 10) and da-GAL4 (da-GAL4/+, n = 12) controls (B). C–F, Effects of temporal knockdown of DopEcR expression on ethanol-induced sedation. Flies were either reared at constant temperature (C, 18°C; D, 29°C) or transferred to a different temperature after eclosion (E, 29°C → 18°C; F, 18°C → 29°C). Adult-specific knockdown of DopEcR expression resulted in increased resistance to ethanol-induced sedation compared with RNAi and da-GAL4 controls; UAS-DopEcR-RNAi/+;da-GAL4/tub-GAL80^ts (n = 11, 12, 11, 3 for C–F), UAS-DopEcR-RNAi/+;tub-GAL80^ts/+ (n = 9, 10, 12, 8), and da-GAL4/+ (n = 8, 7, 6, 6). All figures display averages with SEM, with two-way repeated-measures ANOVA on ranks, Holm–Sidak (A and B, left) and Mann–Whitney rank sum tests (A and B, right; C–F). #p < 0.05 from RNAi and da-GAL4 controls and *p < 0.05 from da-GAL4 control.