Confocal photomicrographs of BDA-labeled and ChAT-expressing cells. A, Diagram of BDA tracer injection in the MC. B, BDA-labeled cells located in the MC near the injection site (arrow). Scale bar, 30 μm. The photomicrograph is a 2D projection of 24 consecutive focal planes located 1 μm apart. C, FN Mns expressing ChAT. Scale bar, 100 μm. D–F, ChAT-expressing FN Mns (magenta) and fibers labeled with BDA (green). Arrows indicate axons anterogradely labeled with BDA. Scale bar: (in F) D–F, 50 μm. Insets in D–F are enlarged (30%) views of labeled FN Mns and MC projecting axons. Photomicrographs are 2D projections of 29 consecutive focal planes located 1 μm apart. G–I, Same ChAT-expressing cells indicated in D–F with arrowheads. Note the axons anterogradely labeled with BDA near or closely apposed to ChAT-expressing FN Mns. Scale bar: (in I) G–I, 25 μm. Photomicrographs are 2D projections of 22 consecutive focal planes located 0.5 μm apart.