In vitro FSCV of DA release and metabolism in OB slices. A, FSCV detection of DA release evoked by optical stimulation (blue bar) of ChR2-expressing SACs. Plot shows current in pseudo-color versus applied potential (ordinate) and time (abscissa). Oxidative DA current in green. Inset, The voltammogram at the DA voltage potential. B, The COMT inhibitor, tolcapone (10 μm), increases DA signal. C, FSCV DA oxidation current from (A) control and from (B) tolcapone. D, Average graph of the peak DA oxidative current in control conditions and in the presence of tolcapone. E, FSCV detection of DA release evoked by optical stimulation (blue bar) of ChR2-expressing SACs. F, The DAT inhibitor, GBR12909 (5 μm), does not alter the DA signal. G, FSCV DA oxidation current from (A) control and from (B) GBR12909. H, Average graph of the peak DA oxidative current in control conditions and in the presence of GBR12909.